This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6. [provided by RefSeq, Mar 2012]
Transcription factors with Perturb-seq knockdown data for BTRC. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = BTRC upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of BTRC, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:101,060,073–101,063,211 | 291.4 kb | Distal (>10kb) Multiome | 1124 | |
| chr10:101,066,181–101,066,983 | 287.6 kb | Distal (>10kb) Multiome | 734 | |
| chr10:101,067,105–101,068,123 | 286.3 kb | Distal (>10kb) Multiome | 643 | |
| chr10:101,095,368–101,096,269 | 258.4 kb | Distal (>10kb) Multiome | 326 | |
| chr10:101,131,094–101,132,387 | 222.8 kb | Distal (>10kb) Multiome | 289 | |
| chr10:101,139,407–101,140,520 | 214.4 kb | Distal (>10kb) Multiome | 158 | |
| chr10:101,161,678–101,162,270 | 192.3 kb | Distal (>10kb) Multiome | 336 | |
| chr10:101,214,601–101,215,378 | 139.2 kb | Distal (>10kb) Multiome | 302 | |
| chr10:101,217,392–101,218,117 | 136.5 kb | Distal (>10kb) Multiome | 203 | |
| chr10:101,236,527–101,237,332 | 117.2 kb | Distal (>10kb) Multiome | 123 | |
| chr10:101,282,747–101,283,297 | 71.1 kb | Distal (>10kb) Multiome | 74 | |
| chr10:101,285,534–101,286,046 | 68.3 kb | Distal (>10kb) Multiome | 150 | |
| chr10:101,288,327–101,289,007 | 65.5 kb | Distal (>10kb) Multiome | 96 | |
| chr10:101,291,317–101,293,346 | 62.4 kb | Distal (>10kb) Multiome | 433 | |
| chr10:101,311,194–101,311,678 | 42.9 kb | Distal (>10kb) Multiome | 94 | |
| chr10:101,353,481–101,354,935 | 124 bp | At TSS Multiome | 875 | |
| chr10:101,450,379–101,450,896 | 96.5 kb | Distal (>10kb) Multiome | 226 | |
| chr10:101,569,467–101,571,017 | 216.1 kb | Distal (>10kb) Multiome | 558 | |
| chr10:101,587,742–101,588,624 | 234.1 kb | Distal (>10kb) Multiome | 673 | |
| chr10:101,602,717–101,603,282 | 248.8 kb | Distal (>10kb) Multiome | 348 | |
| chr10:101,606,618–101,607,814 | 253.1 kb | Distal (>10kb) Multiome | 143 |
Genomic view of the BTRC locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.