This gene encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for BTF3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = BTF3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of BTF3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:73,201,724–73,202,388 | 296.3 kb | Distal (>10kb) Multiome | 402 | |
| chr5:73,215,701–73,216,633 | 282.2 kb | Distal (>10kb) Multiome | 817 | |
| chr5:73,298,965–73,299,493 | 199.2 kb | Distal (>10kb) Multiome | 195 | |
| chr5:73,303,035–73,304,047 | 194.8 kb | Distal (>10kb) Multiome | 200 | |
| chr5:73,381,483–73,382,813 | 116.4 kb | Distal (>10kb) Multiome | 174 | |
| chr5:73,419,250–73,420,167 | 78.7 kb | Distal (>10kb) Multiome | 205 | |
| chr5:73,436,362–73,437,853 | 60.9 kb | Distal (>10kb) Multiome | 622 | |
| chr5:73,443,832–73,444,744 | 54.1 kb | Distal (>10kb) Multiome | 212 | |
| chr5:73,446,878–73,449,160 | 49.7 kb | Distal (>10kb) Multiome | 568 | |
| chr5:73,451,190–73,451,907 | 46.9 kb | Distal (>10kb) Multiome HiCAR | 413 | |
| chr5:73,497,594–73,498,924 | 27 bp | At TSS Multiome | 924 | |
| chr5:73,519,532–73,520,021 | 21.4 kb | Distal (>10kb) Multiome | 375 | |
| chr5:73,564,957–73,566,058 | 67.2 kb | Distal (>10kb) Multiome | 828 | |
| chr5:73,625,688–73,626,803 | 127.7 kb | Distal (>10kb) Multiome | 623 | |
| chr5:73,795,627–73,796,633 | 297.6 kb | Distal (>10kb) Multiome | 254 |
Genomic view of the BTF3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.