This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for BRF2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = BRF2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of BRF2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:37,675,185–37,675,906 | 174.4 kb | Distal (>10kb) Multiome | 507 | |
| chr8:37,694,827–37,698,811 | 154.3 kb | Distal (>10kb) Multiome | 830 | |
| chr8:37,735,982–37,737,291 | 113.3 kb | Distal (>10kb) Multiome | 958 | |
| chr8:37,762,144–37,763,270 | 87.3 kb | Distal (>10kb) Multiome | 889 | |
| chr8:37,796,327–37,797,580 | 53.0 kb | Distal (>10kb) Multiome | 485 | |
| chr8:37,801,462–37,802,090 | 48.0 kb | Distal (>10kb) Multiome HiCAR | 164 | |
| chr8:37,821,399–37,822,221 | 28.0 kb | Distal (>10kb) Multiome | 284 | |
| chr8:37,826,056–37,826,968 | 23.2 kb | Distal (>10kb) Multiome | 259 | |
| chr8:37,841,106–37,843,516 | 6.3 kb | Proximal (<10kb) | 523 | |
| chr8:37,849,554–37,850,089 | 34 bp | At TSS Multiome | 929 | |
| chr8:37,851,505–37,851,692 | 1.6 kb | Proximal (<10kb) | 210 | |
| chr8:37,898,759–37,900,024 | 49.7 kb | Distal (>10kb) Multiome HiCAR | 956 | |
| chr8:37,915,980–37,916,519 | 66.5 kb | Distal (>10kb) Multiome HiCAR | 465 | |
| chr8:37,965,216–37,966,371 | 115.8 kb | Distal (>10kb) Multiome | 177 | |
| chr8:38,015,136–38,015,926 | 165.7 kb | Distal (>10kb) Multiome | 59 | |
| chr8:38,029,913–38,031,249 | 180.6 kb | Distal (>10kb) Multiome | 948 | |
| chr8:38,066,984–38,067,582 | 217.4 kb | Distal (>10kb) Multiome HiCAR | 414 | |
| chr8:38,070,928–38,072,232 | 221.9 kb | Distal (>10kb) Multiome | 282 | |
| chr8:38,104,797–38,106,255 | 255.8 kb | Distal (>10kb) Multiome | 954 | |
| chr8:38,108,727–38,109,662 | 259.2 kb | Distal (>10kb) Multiome | 83 |
Genomic view of the BRF2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.