BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for BAG3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = BAG3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of BAG3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:119,542,175–119,543,695 | 108.5 kb | Distal (>10kb) Multiome | 709 | |
| chr10:119,596,043–119,597,595 | 54.3 kb | Distal (>10kb) Multiome | 893 | |
| chr10:119,650,691–119,652,293 | 97 bp | At TSS Multiome | 828 | |
| chr10:119,654,717–119,655,266 | 3.3 kb | Proximal (<10kb) | 143 | |
| chr10:119,656,886–119,657,097 | 5.5 kb | Proximal (<10kb) | 63 | |
| chr10:119,660,009–119,660,666 | 9.0 kb | Proximal (<10kb) Multiome | 146 | |
| chr10:119,686,526–119,688,685 | 36.9 kb | Distal (>10kb) Multiome | 790 | |
| chr10:119,725,081–119,726,842 | 74.6 kb | Distal (>10kb) Multiome | 818 | |
| chr10:119,817,887–119,819,190 | 167.2 kb | Distal (>10kb) Multiome | 334 | |
| chr10:119,872,104–119,873,627 | 221.6 kb | Distal (>10kb) Multiome | 835 | |
| chr10:119,892,259–119,893,097 | 241.2 kb | Distal (>10kb) Multiome | 957 |
Genomic view of the BAG3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.