This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene encodes an enzyme that may be mainly involved in the synthesis of the first N-acetyllactosamine unit of poly-N-acetyllactosamine chains. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2010]
Transcription factors with Perturb-seq knockdown data for B4GALT3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = B4GALT3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of B4GALT3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:160,910,331–160,911,177 | 266.8 kb | Distal (>10kb) Multiome | 97 | |
| chr1:160,947,055–160,947,669 | 230.1 kb | Distal (>10kb) Multiome | 35 | |
| chr1:160,948,561–160,949,851 | 228.1 kb | Distal (>10kb) Multiome | 173 | |
| chr1:160,981,323–160,982,552 | 195.7 kb | Distal (>10kb) Multiome | 156 | |
| chr1:161,020,536–161,021,920 | 156.2 kb | Distal (>10kb) Multiome | 846 | |
| chr1:161,038,489–161,039,130 | 138.6 kb | Distal (>10kb) Multiome | 468 | |
| chr1:161,045,080–161,046,516 | 131.5 kb | Distal (>10kb) Multiome | 927 | |
| chr1:161,097,691–161,099,047 | 79.2 kb | Distal (>10kb) Multiome | 829 | |
| chr1:161,117,504–161,118,419 | 59.4 kb | Distal (>10kb) Multiome | 794 | |
| chr1:161,131,760–161,133,351 | 44.8 kb | Distal (>10kb) Multiome | 956 | |
| chr1:161,153,700–161,154,461 | 23.5 kb | Distal (>10kb) Multiome | 816 | |
| chr1:161,158,928–161,160,167 | 18.0 kb | Distal (>10kb) Multiome | 706 | |
| chr1:161,165,740–161,167,170 | 11.2 kb | Distal (>10kb) Multiome | 888 | |
| chr1:161,176,874–161,177,972 | 3 bp | At TSS Multiome | 910 | |
| chr1:161,198,215–161,199,209 | 21.0 kb | Distal (>10kb) Multiome | 357 | |
| chr1:161,201,572–161,202,783 | 24.8 kb | Distal (>10kb) Multiome | 852 | |
| chr1:161,225,326–161,226,910 | 48.4 kb | Distal (>10kb) Multiome | 710 | |
| chr1:161,227,235–161,227,802 | 50.0 kb | Distal (>10kb) Multiome | 372 | |
| chr1:161,258,515–161,259,098 | 81.3 kb | Distal (>10kb) Multiome | 281 | |
| chr1:161,305,737–161,306,708 | 128.8 kb | Distal (>10kb) Multiome | 430 | |
| chr1:161,313,977–161,314,703 | 136.8 kb | Distal (>10kb) Multiome | 824 | |
| chr1:161,389,675–161,390,784 | 212.6 kb | Distal (>10kb) Multiome | 866 | |
| chr1:161,399,098–161,400,259 | 222.2 kb | Distal (>10kb) Multiome | 1040 |
Genomic view of the B4GALT3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.