This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene synthesizes N-acetyllactosamine in glycolipids and glycoproteins. Its substrate specificity is affected by alpha-lactalbumin but it is not expressed in lactating mammary tissue. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]
Transcription factors with Perturb-seq knockdown data for B4GALT2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = B4GALT2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of B4GALT2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:43,707,108–43,708,162 | 271.6 kb | Distal (>10kb) Multiome | 937 | |
| chr1:43,933,385–43,935,511 | 44.3 kb | Distal (>10kb) Multiome | 565 | |
| chr1:43,935,659–43,937,001 | 42.5 kb | Distal (>10kb) Multiome | 359 | |
| chr1:43,946,199–43,947,337 | 32.5 kb | Distal (>10kb) Multiome | 1003 | |
| chr1:43,969,717–43,970,284 | 9.3 kb | Proximal (<10kb) Multiome | 515 | |
| chr1:43,974,246–43,975,571 | 4.3 kb | Proximal (<10kb) Multiome | 875 | |
| chr1:43,978,661–43,979,381 | 67 bp | At TSS Multiome | 419 | |
| chr1:43,991,242–43,992,448 | 12.7 kb | Distal (>10kb) Multiome | 780 | |
| chr1:44,000,140–44,000,654 | 21.3 kb | Distal (>10kb) Multiome | 43 | |
| chr1:44,029,597–44,032,070 | 52.5 kb | Distal (>10kb) Multiome | 896 | |
| chr1:44,036,004–44,036,357 | 57.0 kb | Distal (>10kb) Multiome | 452 | |
| chr1:44,134,442–44,134,897 | 155.5 kb | Distal (>10kb) Multiome | 32 | |
| chr1:44,213,251–44,213,777 | 234.3 kb | Distal (>10kb) Multiome | 1062 | |
| chr1:44,238,024–44,238,695 | 259.2 kb | Distal (>10kb) Multiome | 396 | |
| chr1:44,240,892–44,241,669 | 262.1 kb | Distal (>10kb) Multiome | 103 |
Genomic view of the B4GALT2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.