This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene is oriented telomere to centromere in close proximity to the ribosomal protein S18 gene. The functionality of the encoded protein is limited to ganglioseries glycolipid biosynthesis. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for B3GALT4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = B3GALT4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of B3GALT4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:33,009,348–33,009,899 | 267.4 kb | Distal (>10kb) Multiome | 184 | |
| chr6:33,080,635–33,081,087 | 196.5 kb | Distal (>10kb) Multiome | 141 | |
| chr6:33,161,505–33,162,100 | 115.4 kb | Distal (>10kb) Multiome | 589 | |
| chr6:33,199,801–33,201,289 | 76.5 kb | Distal (>10kb) Multiome | 1049 | |
| chr6:33,204,263–33,205,422 | 72.5 kb | Distal (>10kb) Multiome | 762 | |
| chr6:33,208,069–33,208,990 | 68.5 kb | Distal (>10kb) Multiome | 858 | |
| chr6:33,248,132–33,249,983 | 28.8 kb | Distal (>10kb) Multiome | 961 | |
| chr6:33,271,370–33,272,508 | 5.2 kb | Proximal (<10kb) Multiome | 1058 | |
| chr6:33,276,685–33,277,905 | 316 bp | At TSS Multiome | 807 | |
| chr6:33,280,035–33,280,319 | 2.9 kb | Proximal (<10kb) | 342 | |
| chr6:33,288,941–33,289,881 | 12.3 kb | Distal (>10kb) Multiome | 701 | |
| chr6:33,297,445–33,300,232 | 20.7 kb | Distal (>10kb) Multiome | 1095 | |
| chr6:33,312,436–33,314,207 | 35.8 kb | Distal (>10kb) Multiome | 795 | |
| chr6:33,317,189–33,318,504 | 40.8 kb | Distal (>10kb) Multiome | 649 | |
| chr6:33,322,636–33,323,832 | 46.0 kb | Distal (>10kb) Multiome | 941 | |
| chr6:33,346,609–33,347,126 | 69.7 kb | Distal (>10kb) Multiome | 37 | |
| chr6:33,390,888–33,392,247 | 114.5 kb | Distal (>10kb) Multiome | 993 | |
| chr6:33,409,936–33,411,502 | 133.4 kb | Distal (>10kb) Multiome | 977 | |
| chr6:33,417,606–33,419,249 | 141.1 kb | Distal (>10kb) Multiome | 973 | |
| chr6:33,419,793–33,420,532 | 143.1 kb | Distal (>10kb) Multiome | 162 | |
| chr6:33,425,590–33,426,362 | 148.8 kb | Distal (>10kb) Multiome | 665 | |
| chr6:33,431,024–33,432,096 | 154.1 kb | Distal (>10kb) Multiome | 337 | |
| chr6:33,454,074–33,454,852 | 177.4 kb | Distal (>10kb) Multiome | 906 | |
| chr6:33,547,185–33,548,324 | 270.6 kb | Distal (>10kb) Multiome | 155 | |
| chr6:33,570,899–33,571,638 | 294.3 kb | Distal (>10kb) Multiome | 755 |
Genomic view of the B3GALT4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.