ATP2B4

ATP2B4

ATPase plasma membrane Ca2+ transporting 4 | PMCA4, ATP2B2, MXRA1

The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Member of: DE-8 DE-8.15 Developmental clusters: GC6

Biological processes 90 terms

ATP binding (GO:0005524)ATP hydrolysis activity (GO:0016887)P-type calcium transporter activity (GO:0005388)P-type calcium transporter activity (GO:0005388)P-type calcium transporter activity (GO:0005388)P-type calcium transporter activity (GO:0005388)PDZ domain binding (GO:0030165)T-tubule (GO:0030315)T-tubule (GO:0030315)Z disc (GO:0030018)basolateral plasma membrane (GO:0016323)calcium ion export (GO:1901660)calcium ion export (GO:1901660)calcium ion import across plasma membrane (GO:0098703)calcium ion transmembrane import into cytosol (GO:0097553)calcium ion transmembrane transport (GO:0070588)calcium ion transmembrane transport (GO:0070588)calcium ion transmembrane transporter activity (GO:0015085)calcium ion transport (GO:0006816)calmodulin binding (GO:0005516)caveola (GO:0005901)cellular response to acetylcholine (GO:1905145)cellular response to acetylcholine (GO:1905145)cellular response to epinephrine stimulus (GO:0071872)flagellated sperm motility (GO:0030317)flagellated sperm motility (GO:0030317)glutamatergic synapse (GO:0098978)hippocampus development (GO:0021766)intracellular calcium ion homeostasis (GO:0006874)intracellular calcium ion homeostasis (GO:0006874)intracellular calcium ion homeostasis (GO:0006874)intracellular calcium ion homeostasis (GO:0006874)intracellular membrane-bounded organelle (GO:0043231)membrane (GO:0016020)membrane (GO:0016020)membrane (GO:0016020)membrane raft (GO:0045121)monoatomic ion transmembrane transport (GO:0034220)negative regulation of adenylate cyclase-activating adrenergic receptor signaling pathway (GO:0071878)negative regulation of angiogenesis (GO:0016525)negative regulation of angiogenesis (GO:0016525)negative regulation of arginine catabolic process (GO:1900082)negative regulation of blood vessel endothelial cell migration (GO:0043537)negative regulation of blood vessel endothelial cell migration (GO:0043537)negative regulation of calcineurin-NFAT signaling cascade (GO:0070885)negative regulation of calcineurin-NFAT signaling cascade (GO:0070885)negative regulation of calcineurin-NFAT signaling cascade (GO:0070885)negative regulation of cardiac muscle hypertrophy in response to stress (GO:1903243)negative regulation of cardiac muscle hypertrophy in response to stress (GO:1903243)negative regulation of cellular response to vascular endothelial growth factor stimulus (GO:1902548)negative regulation of citrulline biosynthetic process (GO:1903249)negative regulation of gene expression (GO:0010629)negative regulation of gene expression (GO:0010629)negative regulation of nitric oxide biosynthetic process (GO:0045019)negative regulation of the force of heart contraction (GO:0098736)neural retina development (GO:0003407)nitric oxide-cGMP-mediated signaling (GO:0038060)nitric-oxide synthase binding (GO:0050998)nitric-oxide synthase inhibitor activity (GO:0036487)nucleotide binding (GO:0000166)plasma membrane (GO:0005886)plasma membrane (GO:0005886)plasma membrane (GO:0005886)plasma membrane (GO:0005886)plasma membrane (GO:0005886)positive regulation of protein localization to plasma membrane (GO:1903078)presynaptic active zone membrane (GO:0048787)protein binding (GO:0005515)protein kinase binding (GO:0019901)protein phosphatase 2B binding (GO:0030346)protein phosphatase 2B binding (GO:0030346)protein phosphatase 2B binding (GO:0030346)protein-containing complex (GO:0032991)regulation of cardiac conduction (GO:1903779)regulation of cardiac conduction (GO:1903779)regulation of cell cycle G1/S phase transition (GO:1902806)regulation of cytosolic calcium ion concentration (GO:0051480)regulation of sodium ion transmembrane transport (GO:1902305)regulation of transcription by RNA polymerase II (GO:0006357)response to hydrostatic pressure (GO:0051599)sarcolemma (GO:0042383)scaffold protein binding (GO:0097110)sodium channel regulator activity (GO:0017080)sperm flagellum (GO:0036126)sperm flagellum (GO:0036126)sperm principal piece (GO:0097228)spermatogenesis (GO:0007283)transport across blood-brain barrier (GO:0150104)urinary bladder smooth muscle contraction (GO:0014832)urinary bladder smooth muscle contraction (GO:0014832)

Expression (TPM)

TFs regulating ATP2B4 0 TFs

Transcription factors with Perturb-seq knockdown data for ATP2B4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ATP2B4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to ATP2B4

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ATP2B4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility	Element	Dist. to TSS	Link type	TFs
	chr1:203,326,875–203,328,263	299.2 kb	Distal (>10kb) Multiome	723
	chr1:203,342,300–203,342,959	284.2 kb	Distal (>10kb) Multiome	242
	chr1:203,369,826–203,371,538	256.5 kb	Distal (>10kb) Multiome	235
	chr1:203,487,217–203,488,252	139.1 kb	Distal (>10kb) Multiome	632
	chr1:203,519,126–203,519,875	107.3 kb	Distal (>10kb) Multiome	283
	chr1:203,585,330–203,586,179	41.1 kb	Distal (>10kb) Multiome	85
	chr1:203,592,048–203,592,904	34.3 kb	Distal (>10kb) Multiome	170
	chr1:203,626,216–203,627,194	81 bp	At TSS Multiome	626
	chr1:203,627,926–203,630,057	1.8 kb	Proximal (<10kb) Multiome	425
	chr1:203,650,175–203,650,835	23.7 kb	Distal (>10kb) Multiome	596
	chr1:203,795,422–203,796,461	168.9 kb	Distal (>10kb) Multiome	784
	chr1:203,861,102–203,862,191	234.7 kb	Distal (>10kb) Multiome	1090

Genome Browser

Genomic view of the ATP2B4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.