Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for ATG10. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ATG10 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ATG10, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:81,749,733–81,752,236 | 220.7 kb | Distal (>10kb) Multiome | 930 | |
| chr5:81,851,320–81,853,363 | 120.1 kb | Distal (>10kb) Multiome | 930 | |
| chr5:81,954,769–81,955,286 | 17.0 kb | Distal (>10kb) Multiome | 190 | |
| chr5:81,968,277–81,968,887 | 3.1 kb | Proximal (<10kb) | 50 | |
| chr5:81,971,083–81,972,642 | 7 bp | At TSS Multiome | 973 | |
| chr5:81,980,686–81,981,205 | 8.7 kb | Proximal (<10kb) | 42 |
Genomic view of the ATG10 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.