Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The protein encoded by this gene may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jun 2013]
Transcription factors with Perturb-seq knockdown data for ARHGEF4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ARHGEF4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ARHGEF4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:130,726,871–130,728,450 | 109.2 kb | Distal (>10kb) Multiome | 465 | |
| chr2:130,755,362–130,756,947 | 80.7 kb | Distal (>10kb) Multiome | 431 | |
| chr2:130,797,289–130,799,132 | 39.0 kb | Distal (>10kb) Multiome | 829 | |
| chr2:130,836,282–130,837,627 | 38 bp | At TSS Multiome | 339 | |
| chr2:130,963,257–130,964,855 | 127.8 kb | Distal (>10kb) Multiome | 287 | |
| chr2:130,966,174–130,967,560 | 129.9 kb | Distal (>10kb) Multiome | 143 | |
| chr2:131,034,253–131,036,833 | 198.1 kb | Distal (>10kb) Multiome | 226 | |
| chr2:131,076,813–131,077,478 | 240.1 kb | Distal (>10kb) Multiome | 139 | |
| chr2:131,092,844–131,094,042 | 256.5 kb | Distal (>10kb) Multiome | 934 | |
| chr2:131,104,647–131,106,790 | 268.4 kb | Distal (>10kb) Multiome | 914 |
Genomic view of the ARHGEF4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.