ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP. It contains a Sec7 domain, which may be responsible for guanine-nucleotide exchange activity and also brefeldin A inhibition. [provided by RefSeq, Aug 2011]
Transcription factors with Perturb-seq knockdown data for ARFGEF1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ARFGEF1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ARFGEF1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:67,061,734–67,062,754 | 281.5 kb | Distal (>10kb) Multiome | 733 | |
| chr8:67,063,914–67,065,235 | 279.4 kb | Distal (>10kb) Multiome | 891 | |
| chr8:67,109,497–67,110,469 | 233.7 kb | Distal (>10kb) Multiome | 396 | |
| chr8:67,339,472–67,339,832 | 3.9 kb | Proximal (<10kb) | 197 | |
| chr8:67,342,811–67,344,446 | 72 bp | At TSS Multiome | 722 | |
| chr8:67,368,913–67,370,235 | 25.4 kb | Distal (>10kb) Multiome | 179 | |
| chr8:67,719,621–67,721,354 | 376.6 kb | Distal (>10kb) Multiome HiCAR | 273 |
Genomic view of the ARFGEF1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.