This gene encodes a member of the anoctamin family of transmembrane proteins. The encoded protein is likely a calcium activated chloride channel. Mutations in this gene have been associated with gnathodiaphyseal dysplasia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]
Transcription factors with Perturb-seq knockdown data for ANO5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ANO5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ANO5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:22,187,730–22,187,877 | 5.2 kb | Proximal (<10kb) | 3 | |
| chr11:22,192,093–22,194,240 | 102 bp | At TSS Multiome | 573 | |
| chr11:22,340,758–22,341,768 | 148.1 kb | Distal (>10kb) Multiome | 166 | |
| chr11:22,342,578–22,344,429 | 150.6 kb | Distal (>10kb) Multiome | 210 | |
| chr11:22,432,134–22,433,214 | 239.6 kb | Distal (>10kb) Multiome | 266 |
Genomic view of the ANO5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.