Transcription factors with Perturb-seq knockdown data for ANKRD44. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ANKRD44 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ANKRD44, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:197,143,874–197,144,411 | 166.6 kb | Distal (>10kb) Multiome HiCAR | 70 | |
| chr2:197,147,612–197,148,468 | 162.8 kb | Distal (>10kb) Multiome HiCAR | 159 | |
| chr2:197,309,737–197,311,360 | 56 bp | At TSS Multiome | 642 | |
| chr2:197,322,642–197,323,971 | 12.6 kb | Distal (>10kb) Multiome | 185 | |
| chr2:197,434,572–197,435,653 | 124.3 kb | Distal (>10kb) Multiome | 937 | |
| chr2:197,452,830–197,454,321 | 142.6 kb | Distal (>10kb) Multiome | 1033 | |
| chr2:197,498,722–197,500,854 | 189.3 kb | Distal (>10kb) Multiome | 1173 | |
| chr2:197,515,482–197,516,752 | 205.2 kb | Distal (>10kb) Multiome | 1138 |
Genomic view of the ANKRD44 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.