This gene encodes a member of the LEM family of inner nuclear membrane proteins. The encoded protein functions as a mitotic regulator through postmitotic formation of the nuclear envelope. Mutations in this gene cause morphology defects in the nuclear envelope and BAF hyperphosphorylation. [provided by RefSeq, Mar 2014]
Transcription factors with Perturb-seq knockdown data for ANKLE2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ANKLE2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ANKLE2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:132,488,556–132,491,043 | 271.8 kb | Distal (>10kb) Multiome | 563 | |
| chr12:132,559,692–132,561,628 | 201.8 kb | Distal (>10kb) Multiome | 607 | |
| chr12:132,598,373–132,599,027 | 163.3 kb | Distal (>10kb) Multiome | 242 | |
| chr12:132,618,319–132,618,996 | 143.2 kb | Distal (>10kb) Multiome | 202 | |
| chr12:132,686,665–132,688,078 | 74.4 kb | Distal (>10kb) Multiome | 824 | |
| chr12:132,710,174–132,711,444 | 51.1 kb | Distal (>10kb) Multiome | 940 | |
| chr12:132,761,549–132,762,490 | 250 bp | At TSS Multiome | 760 | |
| chr12:132,768,489–132,768,709 | 6.7 kb | Proximal (<10kb) | 251 | |
| chr12:132,828,453–132,829,499 | 67.3 kb | Distal (>10kb) Multiome | 930 | |
| chr12:132,887,106–132,888,371 | 125.9 kb | Distal (>10kb) Multiome | 840 | |
| chr12:132,907,962–132,909,233 | 146.8 kb | Distal (>10kb) Multiome | 481 | |
| chr12:132,955,400–132,956,621 | 194.5 kb | Distal (>10kb) Multiome | 715 | |
| chr12:132,986,063–132,986,840 | 224.5 kb | Distal (>10kb) Multiome | 840 | |
| chr12:133,036,951–133,037,971 | 275.6 kb | Distal (>10kb) Multiome | 973 |
Genomic view of the ANKLE2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.