Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact, and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 3 is an immunologically distinct gene product from ankyrins 1 and 2, and was originally found at the axonal initial segment and nodes of Ranvier of neurons in the central and peripheral nervous systems. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]
Transcription factors with Perturb-seq knockdown data for ANK3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ANK3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ANK3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:60,732,197–60,733,762 | 58 bp | At TSS Multiome | 435 | |
| chr10:60,733,866–60,734,461 | 376 bp | At TSS | 79 | |
| chr10:60,735,187–60,735,673 | 1.7 kb | Proximal (<10kb) | 54 | |
| chr10:60,742,442–60,742,853 | 9.0 kb | Proximal (<10kb) | 86 | |
| chr10:60,777,960–60,779,192 | 45.0 kb | Distal (>10kb) Multiome HiCAR | 870 | |
| chr10:60,942,933–60,945,250 | 210.9 kb | Distal (>10kb) Multiome | 836 | |
| chr10:61,000,729–61,002,143 | 267.9 kb | Distal (>10kb) Multiome | 629 |
Genomic view of the ANK3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.