This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015]
Transcription factors with Perturb-seq knockdown data for AGO1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AGO1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AGO1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:35,641,114–35,641,872 | 241.6 kb | Distal (>10kb) Multiome | 824 | |
| chr1:35,707,208–35,708,693 | 175.3 kb | Distal (>10kb) Multiome | 388 | |
| chr1:35,717,979–35,719,281 | 164.3 kb | Distal (>10kb) Multiome | 558 | |
| chr1:35,769,208–35,770,406 | 113.2 kb | Distal (>10kb) Multiome | 929 | |
| chr1:35,807,537–35,808,824 | 75.2 kb | Distal (>10kb) Multiome | 575 | |
| chr1:35,882,591–35,884,430 | 84 bp | At TSS Multiome | 802 | |
| chr1:35,885,650–35,886,507 | 3.0 kb | Proximal (<10kb) Multiome | 156 | |
| chr1:35,930,610–35,931,886 | 47.8 kb | Distal (>10kb) Multiome | 903 | |
| chr1:36,083,788–36,084,510 | 201.0 kb | Distal (>10kb) Multiome | 194 | |
| chr1:36,088,326–36,089,393 | 205.7 kb | Distal (>10kb) Multiome | 1023 | |
| chr1:36,148,864–36,150,075 | 266.3 kb | Distal (>10kb) Multiome | 925 | |
| chr1:36,150,344–36,151,320 | 267.8 kb | Distal (>10kb) Multiome | 524 | |
| chr1:36,155,033–36,157,490 | 272.8 kb | Distal (>10kb) Multiome | 790 | |
| chr1:36,166,478–36,167,190 | 283.6 kb | Distal (>10kb) Multiome | 85 | |
| chr1:36,176,148–36,176,823 | 293.2 kb | Distal (>10kb) Multiome | 141 | |
| chr1:36,178,119–36,179,461 | 295.8 kb | Distal (>10kb) Multiome | 222 |
Genomic view of the AGO1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.