This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for ACTL6A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ACTL6A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ACTL6A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:179,322,380–179,323,876 | 240.0 kb | Distal (>10kb) Multiome | 958 | |
| chr3:179,346,338–179,348,224 | 215.3 kb | Distal (>10kb) Multiome HiCAR | 843 | |
| chr3:179,450,888–179,452,034 | 111.4 kb | Distal (>10kb) Multiome | 580 | |
| chr3:179,504,159–179,505,204 | 58.4 kb | Distal (>10kb) Multiome | 128 | |
| chr3:179,526,783–179,527,418 | 35.9 kb | Distal (>10kb) Multiome | 358 | |
| chr3:179,558,850–179,559,030 | 3.9 kb | Proximal (<10kb) | 135 | |
| chr3:179,562,395–179,564,138 | 86 bp | At TSS Multiome | 866 | |
| chr3:179,604,200–179,605,304 | 41.8 kb | Distal (>10kb) Multiome | 786 | |
| chr3:179,608,248–179,609,637 | 45.9 kb | Distal (>10kb) Multiome | 176 | |
| chr3:179,652,424–179,653,975 | 90.1 kb | Distal (>10kb) Multiome | 805 |
Genomic view of the ACTL6A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.