This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]
Transcription factors with Perturb-seq knockdown data for ACSS2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ACSS2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ACSS2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:34,676,509–34,677,593 | 199.6 kb | Distal (>10kb) Multiome | 954 | |
| chr20:34,703,810–34,705,103 | 172.1 kb | Distal (>10kb) Multiome | 903 | |
| chr20:34,708,877–34,710,404 | 166.8 kb | Distal (>10kb) Multiome | 576 | |
| chr20:34,824,452–34,825,860 | 51.1 kb | Distal (>10kb) Multiome | 852 | |
| chr20:34,872,384–34,873,259 | 3.7 kb | Proximal (<10kb) Multiome | 817 | |
| chr20:34,874,110–34,874,535 | 2.3 kb | Proximal (<10kb) Multiome | 357 | |
| chr20:34,875,878–34,877,081 | 190 bp | At TSS Multiome | 852 | |
| chr20:34,955,039–34,956,224 | 79.2 kb | Distal (>10kb) Multiome HiCAR | 1031 | |
| chr20:34,960,537–34,961,054 | 84.3 kb | Distal (>10kb) Multiome HiCAR | 275 | |
| chr20:34,979,568–34,980,289 | 103.3 kb | Distal (>10kb) Multiome HiCAR | 149 | |
| chr20:35,092,320–35,093,398 | 216.2 kb | Distal (>10kb) Multiome | 1139 | |
| chr20:35,146,563–35,147,734 | 270.7 kb | Distal (>10kb) Multiome | 992 | |
| chr20:35,171,731–35,172,358 | 295.4 kb | Distal (>10kb) Multiome | 383 | |
| chr20:35,174,680–35,175,277 | 298.4 kb | Distal (>10kb) Multiome | 635 | |
| chr20:35,262,839–35,263,582 | 386.7 kb | Distal (>10kb) Multiome HiCAR | 336 | |
| chr20:35,269,197–35,269,766 | 392.9 kb | Distal (>10kb) Multiome HiCAR | 447 | |
| chr20:35,277,415–35,278,519 | 401.5 kb | Distal (>10kb) Multiome HiCAR | 575 |
Genomic view of the ACSS2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.