Short/branched chain acyl-CoA dehydrogenase(ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield a mature peptide of approximately 43.7-KDa. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for ACADSB. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ACADSB upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ACADSB, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:122,875,885–122,876,836 | 132.7 kb | Distal (>10kb) Multiome | 128 | |
| chr10:122,877,140–122,877,717 | 131.5 kb | Distal (>10kb) Multiome | 112 | |
| chr10:122,879,124–122,880,264 | 129.2 kb | Distal (>10kb) Multiome | 712 | |
| chr10:122,953,726–122,954,686 | 54.7 kb | Distal (>10kb) Multiome | 995 | |
| chr10:122,980,093–122,981,015 | 28.5 kb | Distal (>10kb) Multiome | 874 | |
| chr10:123,007,898–123,009,768 | 66 bp | At TSS Multiome | 911 | |
| chr10:123,017,527–123,017,748 | 8.5 kb | Proximal (<10kb) | 143 | |
| chr10:123,017,895–123,018,046 | 8.9 kb | Proximal (<10kb) | 15 | |
| chr10:123,132,736–123,133,704 | 124.2 kb | Distal (>10kb) Multiome | 384 | |
| chr10:123,135,104–123,137,885 | 128.3 kb | Distal (>10kb) Multiome | 563 | |
| chr10:123,139,520–123,140,375 | 131.0 kb | Distal (>10kb) Multiome | 456 | |
| chr10:123,147,570–123,148,872 | 139.1 kb | Distal (>10kb) Multiome | 310 | |
| chr10:123,150,788–123,151,726 | 142.2 kb | Distal (>10kb) Multiome | 224 | |
| chr10:123,153,975–123,155,184 | 145.3 kb | Distal (>10kb) Multiome | 787 |
Genomic view of the ACADSB locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.