This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms. [provided by RefSeq, Oct 2014]
Transcription factors with Perturb-seq knockdown data for ACADS. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ACADS upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ACADS, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:120,437,436–120,438,568 | 287.8 kb | Distal (>10kb) Multiome | 725 | |
| chr12:120,445,927–120,446,970 | 279.3 kb | Distal (>10kb) Multiome | 982 | |
| chr12:120,468,864–120,470,390 | 256.0 kb | Distal (>10kb) Multiome | 942 | |
| chr12:120,495,362–120,496,939 | 229.8 kb | Distal (>10kb) Multiome | 794 | |
| chr12:120,528,856–120,529,468 | 196.6 kb | Distal (>10kb) Multiome | 849 | |
| chr12:120,534,230–120,535,077 | 191.3 kb | Distal (>10kb) Multiome | 730 | |
| chr12:120,580,919–120,581,830 | 144.3 kb | Distal (>10kb) Multiome | 736 | |
| chr12:120,584,329–120,585,045 | 141.0 kb | Distal (>10kb) Multiome | 460 | |
| chr12:120,640,005–120,641,704 | 85.3 kb | Distal (>10kb) Multiome | 562 | |
| chr12:120,648,914–120,649,665 | 76.5 kb | Distal (>10kb) Multiome | 405 | |
| chr12:120,686,185–120,687,741 | 39.0 kb | Distal (>10kb) Multiome | 1030 | |
| chr12:120,710,025–120,711,277 | 15.4 kb | Distal (>10kb) Multiome HiCAR | 573 | |
| chr12:120,725,271–120,726,309 | 58 bp | At TSS Multiome | 663 | |
| chr12:120,822,842–120,823,717 | 97.4 kb | Distal (>10kb) Multiome | 117 | |
| chr12:120,903,538–120,904,786 | 178.6 kb | Distal (>10kb) Multiome | 749 | |
| chr12:121,016,022–121,016,705 | 290.7 kb | Distal (>10kb) Multiome | 674 |
Genomic view of the ACADS locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.