This gene encodes a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. The encoded protein is a mitochondrial enzyme that functions in catabolism of the branched-chain amino acid valine. Defects in this gene are the cause of isobutyryl-CoA dehydrogenase deficiency.[provided by RefSeq, Nov 2009]
Transcription factors with Perturb-seq knockdown data for ACAD8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ACAD8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ACAD8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:133,955,545–133,957,834 | 296.5 kb | Distal (>10kb) Multiome | 423 | |
| chr11:134,033,701–134,034,640 | 219.4 kb | Distal (>10kb) Multiome | 251 | |
| chr11:134,036,538–134,037,918 | 216.3 kb | Distal (>10kb) Multiome | 381 | |
| chr11:134,068,849–134,069,824 | 184.4 kb | Distal (>10kb) Multiome | 407 | |
| chr11:134,223,679–134,225,624 | 28.9 kb | Distal (>10kb) Multiome | 944 | |
| chr11:134,252,809–134,254,050 | 118 bp | At TSS Multiome | 758 | |
| chr11:134,262,118–134,262,300 | 8.6 kb | Proximal (<10kb) | 103 | |
| chr11:134,272,860–134,273,509 | 19.6 kb | Distal (>10kb) Multiome | 183 | |
| chr11:134,275,655–134,277,823 | 22.9 kb | Distal (>10kb) Multiome | 418 | |
| chr11:134,331,384–134,332,745 | 78.3 kb | Distal (>10kb) Multiome | 483 | |
| chr11:134,360,067–134,360,637 | 106.9 kb | Distal (>10kb) Multiome | 397 | |
| chr11:134,411,283–134,413,029 | 158.7 kb | Distal (>10kb) Multiome | 258 |
Genomic view of the ACAD8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.