The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for AARS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AARS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AARS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:70,065,697–70,066,412 | 223.5 kb | Distal (>10kb) Multiome | 331 | |
| chr16:70,114,104–70,114,595 | 175.2 kb | Distal (>10kb) Multiome | 324 | |
| chr16:70,250,987–70,252,565 | 37.6 kb | Distal (>10kb) Multiome | 1128 | |
| chr16:70,288,832–70,289,863 | 17 bp | At TSS Multiome | 1002 | |
| chr16:70,299,053–70,299,260 | 9.5 kb | Proximal (<10kb) | 740 | |
| chr16:70,299,420–70,299,588 | 9.9 kb | Proximal (<10kb) | 657 | |
| chr16:70,346,498–70,347,297 | 57.3 kb | Distal (>10kb) Multiome | 945 | |
| chr16:70,380,270–70,382,056 | 92.0 kb | Distal (>10kb) Multiome | 1036 | |
| chr16:70,415,208–70,415,782 | 125.9 kb | Distal (>10kb) Multiome | 229 | |
| chr16:70,427,766–70,429,282 | 138.9 kb | Distal (>10kb) Multiome | 981 | |
| chr16:70,431,040–70,431,529 | 141.6 kb | Distal (>10kb) Multiome | 302 | |
| chr16:70,436,957–70,437,432 | 147.7 kb | Distal (>10kb) Multiome | 457 | |
| chr16:70,438,422–70,439,873 | 149.7 kb | Distal (>10kb) Multiome | 926 | |
| chr16:70,454,276–70,454,976 | 165.0 kb | Distal (>10kb) Multiome | 845 | |
| chr16:70,457,673–70,458,151 | 168.4 kb | Distal (>10kb) Multiome | 38 | |
| chr16:70,523,103–70,524,281 | 234.2 kb | Distal (>10kb) Multiome | 903 |
Genomic view of the AARS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.