Predicted to enable DNA binding activity and DNA-binding transcription factor activity, RNA polymerase II-specific. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for ZNF654. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZNF654 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZNF654, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:87,226,657–87,228,058 | 832.0 kb | Distal (>10kb) Multiome HiCAR | 799 | |
| chr3:87,792,241–87,793,786 | 266.3 kb | Distal (>10kb) Multiome | 465 | |
| chr3:88,024,532–88,025,618 | 34.1 kb | Distal (>10kb) Multiome | 89 | |
| chr3:88,058,031–88,060,101 | 175 bp | At TSS Multiome | 892 | |
| chr3:88,149,147–88,150,399 | 90.7 kb | Distal (>10kb) Multiome | 973 |
Genomic view of the ZNF654 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.