The protein encoded by this gene is a zinc finger transcription factor that may play a role in eye development. Defects in this gene have been associated with high myopia. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Modules significantly affected by knockdown. ↑ Up = module upregulated upon KD; ↓ Down = module downregulated upon KD.
| Cluster | Dir | NES | padj | Bind | OR | padj (bind) |
|---|
| Module | Dir | NES | #gRNA | padj | Bind | OR | padj (bind) |
|---|
| Submodule | Module | Dir | NES | #gRNA | Bind | OR | padj (bind) |
|---|
Genes likely regulated by ZNF644 through linked binding evidence in open chromatin. The chart ranks TF-linked genes by their mean Perturb-seq response to ZNF644 knockdown, with negative coefficients indicating downregulation and positive coefficients indicating upregulation upon knockdown.
Open chromatin elements (ATAC-seq) where ZNF644 has ChIP-seq or motif footprint binding evidence and which are linked to at least one target gene region.
| Element | Size | Linked genes |
|---|
Transcription factors with Perturb-seq knockdown data for ZNF644. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZNF644 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZNF644, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:90,723,557–90,724,199 | 298.4 kb | Distal (>10kb) Multiome HiCAR | 186 | |
| chr1:90,729,370–90,729,963 | 292.6 kb | Distal (>10kb) Multiome HiCAR | 164 | |
| chr1:90,833,266–90,836,598 | 187.3 kb | Distal (>10kb) Multiome | 374 | |
| chr1:90,850,622–90,852,216 | 170.7 kb | Distal (>10kb) Multiome | 712 | |
| chr1:91,021,015–91,022,673 | 504 bp | At TSS Multiome | 1058 | |
| chr1:91,164,910–91,166,050 | 143.1 kb | Distal (>10kb) Multiome | 324 |
Genomic view of the ZNF644 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.