This gene encodes a member of the Krueppel C2H2-type zinc-finger family of proteins. The encoded protein represses transcription of the aldolase A gene, which encodes a key enzyme in glycolysis. The encoded zinc-finger protein may also function as a transcriptional co-activator with Wilms' tumor protein 1 to regulate apoptotic genes in leukemia. [provided by RefSeq, Jul 2016]
Transcription factors with Perturb-seq knockdown data for ZNF224. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZNF224 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZNF224, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:43,798,557–43,799,046 | 295.6 kb | Distal (>10kb) Multiome | 403 | |
| chr19:43,827,133–43,827,702 | 267.1 kb | Distal (>10kb) Multiome | 593 | |
| chr19:43,901,409–43,901,966 | 192.6 kb | Distal (>10kb) Multiome | 528 | |
| chr19:43,934,900–43,935,680 | 159.1 kb | Distal (>10kb) Multiome | 775 | |
| chr19:43,950,951–43,951,533 | 143.2 kb | Distal (>10kb) Multiome | 758 | |
| chr19:43,983,881–43,984,809 | 110.2 kb | Distal (>10kb) Multiome | 634 | |
| chr19:44,002,334–44,003,562 | 91.5 kb | Distal (>10kb) Multiome | 726 | |
| chr19:44,025,169–44,025,638 | 69.0 kb | Distal (>10kb) Multiome | 531 | |
| chr19:44,051,566–44,052,404 | 42.5 kb | Distal (>10kb) Multiome | 706 | |
| chr19:44,071,747–44,072,437 | 22.3 kb | Distal (>10kb) Multiome | 678 | |
| chr19:44,093,936–44,095,205 | 80 bp | At TSS Multiome | 671 | |
| chr19:44,112,896–44,114,096 | 18.9 kb | Distal (>10kb) Multiome | 719 | |
| chr19:44,141,264–44,142,208 | 47.2 kb | Distal (>10kb) Multiome | 705 | |
| chr19:44,164,531–44,165,427 | 70.6 kb | Distal (>10kb) Multiome | 754 | |
| chr19:44,207,320–44,207,849 | 113.1 kb | Distal (>10kb) Multiome | 743 | |
| chr19:44,212,226–44,213,003 | 118.1 kb | Distal (>10kb) Multiome | 758 | |
| chr19:44,259,722–44,260,198 | 165.5 kb | Distal (>10kb) Multiome | 622 | |
| chr19:44,304,672–44,305,404 | 210.6 kb | Distal (>10kb) Multiome | 703 | |
| chr19:44,356,364–44,357,093 | 262.3 kb | Distal (>10kb) Multiome | 406 |
Genomic view of the ZNF224 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.