The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 1 of this gene family. In addition to forming homodimers, this protein heterodimerizes with members 2 and 3 of the zinc fingers and homeoboxes family. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream chromosome 8 open reading frame 76 (C8orf76) gene. [provided by RefSeq, Feb 2011]
Transcription factors with Perturb-seq knockdown data for ZHX1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZHX1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZHX1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:123,041,536–123,042,890 | 232.0 kb | Distal (>10kb) Multiome | 925 | |
| chr8:123,065,582–123,066,386 | 208.2 kb | Distal (>10kb) Multiome | 354 | |
| chr8:123,072,367–123,073,561 | 201.6 kb | Distal (>10kb) Multiome | 858 | |
| chr8:123,156,604–123,157,953 | 117.0 kb | Distal (>10kb) Multiome | 1083 | |
| chr8:123,160,441–123,161,547 | 113.6 kb | Distal (>10kb) Multiome | 590 | |
| chr8:123,205,121–123,205,789 | 68.8 kb | Distal (>10kb) Multiome | 298 | |
| chr8:123,207,081–123,207,716 | 66.9 kb | Distal (>10kb) Multiome | 96 | |
| chr8:123,235,684–123,236,295 | 38.3 kb | Distal (>10kb) Multiome | 110 | |
| chr8:123,240,791–123,241,581 | 32.9 kb | Distal (>10kb) Multiome | 784 | |
| chr8:123,273,286–123,275,625 | 448 bp | At TSS Multiome | 970 | |
| chr8:123,395,612–123,397,193 | 122.3 kb | Distal (>10kb) Multiome | 1096 | |
| chr8:123,416,006–123,417,726 | 142.3 kb | Distal (>10kb) Multiome | 823 | |
| chr8:123,479,357–123,480,359 | 205.5 kb | Distal (>10kb) Multiome | 179 | |
| chr8:123,539,997–123,542,004 | 267.2 kb | Distal (>10kb) Multiome | 699 |
Genomic view of the ZHX1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.