Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be located in chromatin. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for ZFHX4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZFHX4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZFHX4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:76,672,475–76,674,701 | 6.5 kb | Proximal (<10kb) | 486 | |
| chr8:76,676,342–76,676,607 | 4.6 kb | Proximal (<10kb) | 40 | |
| chr8:76,677,304–76,678,556 | 3.2 kb | Proximal (<10kb) Multiome | 396 | |
| chr8:76,680,533–76,681,577 | 116 bp | At TSS Multiome | 458 | |
| chr8:76,681,696–76,683,728 | 458 bp | At TSS | 340 | |
| chr8:76,683,818–76,684,005 | 2.6 kb | Proximal (<10kb) | 77 | |
| chr8:76,686,690–76,687,500 | 5.5 kb | Proximal (<10kb) | 148 | |
| chr8:76,766,926–76,767,474 | 8.9 kb | Proximal (<10kb) | 15 | |
| chr8:76,775,175–76,776,716 | 94.5 kb | Distal (>10kb) Multiome | 319 | |
| chr8:76,778,120–76,778,440 | 1.7 kb | Proximal (<10kb) | 34 | |
| chr8:76,840,609–76,842,259 | 160.8 kb | Distal (>10kb) Multiome | 283 | |
| chr8:76,999,674–77,001,166 | 319.1 kb | Distal (>10kb) Multiome HiCAR | 944 | |
| chr8:78,279,251–78,280,374 | 1598.6 kb | Distal (>10kb) Multiome HiCAR | 154 |
Genomic view of the ZFHX4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.