ZAP70
zeta chain of T cell receptor associated protein kinase 70 | STD, ZAP-70, SRK

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Developmental clusters: GC6
Biological processes 52 terms
ATP binding (GO:0005524)ATP binding (GO:0005524)B cell activation (GO:0042113)T cell activation (GO:0042110)T cell activation (GO:0042110)T cell aggregation (GO:0070489)T cell differentiation (GO:0030217)T cell migration (GO:0072678)T cell receptor complex (GO:0042101)T cell receptor complex (GO:0042101)T cell receptor signaling pathway (GO:0050852)T cell receptor signaling pathway (GO:0050852)T cell receptor signaling pathway (GO:0050852)adaptive immune response (GO:0002250)cell-cell junction (GO:0005911)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytosol (GO:0005829)cytosol (GO:0005829)immune response (GO:0006955)immunological synapse (GO:0001772)intracellular signal transduction (GO:0035556)intracellular signal transduction (GO:0035556)leukocyte cell-cell adhesion (GO:0007159)leukocyte migration (GO:0050900)membrane (GO:0016020)membrane raft (GO:0045121)non-membrane spanning protein tyrosine kinase activity (GO:0004715)non-membrane spanning protein tyrosine kinase activity (GO:0004715)non-membrane spanning protein tyrosine kinase activity (GO:0004715)peptidyl-tyrosine phosphorylation (GO:0018108)peptidyl-tyrosine phosphorylation (GO:0018108)phosphotyrosine residue binding (GO:0001784)plasma membrane (GO:0005886)plasma membrane (GO:0005886)plasma membrane (GO:0005886)plasma membrane (GO:0005886)plasma membrane (GO:0005886)positive regulation of T cell differentiation (GO:0045582)positive regulation of T cell differentiation (GO:0045582)positive thymic T cell selection (GO:0045059)positive thymic T cell selection (GO:0045059)protein binding (GO:0005515)protein kinase activity (GO:0004672)protein phosphorylation (GO:0006468)protein phosphorylation (GO:0006468)protein tyrosine kinase activity (GO:0004713)protein tyrosine kinase activity (GO:0004713)protein tyrosine kinase activity (GO:0004713)protein tyrosine kinase activity (GO:0004713)protein tyrosine kinase activity (GO:0004713)
Expression (TPM)
ZAP70 — as a Regulated Gene

TFs regulating ZAP70 0 TFs

Transcription factors with Perturb-seq knockdown data for ZAP70. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ZAP70 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to ZAP70

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ZAP70, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr2:97,709,962–97,710,175 3.4 kb Proximal (<10kb) 172
chr2:97,711,067–97,711,344 2.2 kb Proximal (<10kb) 100
chr2:97,711,706–97,712,616 959 bp At TSS 160

Genome Browser

Genomic view of the ZAP70 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr2:97,699,962 – 97,722,616
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq