This gene is located upstream of the Wilms tumor 1 (WT1) gene; these two genes are bi-directionally transcribed from the same promoter region. This gene is imprinted in kidney, with preferential expression from the paternal allele. Imprinting defects at chromosome 11p13 may contribute to tumorigenesis. [provided by RefSeq, May 2014]
Transcription factors with Perturb-seq knockdown data for WT1-AS. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = WT1-AS upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of WT1-AS, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:32,426,716–32,427,613 | 8.0 kb | Proximal (<10kb) | 227 | |
| chr11:32,427,790–32,428,346 | 7.3 kb | Proximal (<10kb) | 71 | |
| chr11:32,428,452–32,428,633 | 7.0 kb | Proximal (<10kb) | 39 | |
| chr11:32,430,083–32,430,317 | 5.3 kb | Proximal (<10kb) | 24 | |
| chr11:32,430,504–32,431,526 | 4.1 kb | Proximal (<10kb) | 180 | |
| chr11:32,433,949–32,436,637 | at TSS | At TSS | 544 | |
| chr11:32,436,719–32,437,235 | 1.1 kb | Proximal (<10kb) | 58 | |
| chr11:32,437,732–32,438,388 | 2.1 kb | Proximal (<10kb) | 155 | |
| chr11:32,438,809–32,439,407 | 3.2 kb | Proximal (<10kb) | 120 | |
| chr11:32,441,013–32,441,260 | 5.4 kb | Proximal (<10kb) | 49 |
Genomic view of the WT1-AS locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.