This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]
Transcription factors with Perturb-seq knockdown data for WT1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = WT1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of WT1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:32,426,716–32,427,613 | 3.2 kb | Proximal (<10kb) | 227 | |
| chr11:32,427,790–32,428,346 | 2.5 kb | Proximal (<10kb) | 71 | |
| chr11:32,428,452–32,428,633 | 2.2 kb | Proximal (<10kb) | 39 | |
| chr11:32,430,083–32,430,317 | 495 bp | At TSS | 24 | |
| chr11:32,430,504–32,431,526 | at TSS | At TSS | 180 | |
| chr11:32,433,949–32,436,637 | 3.1 kb | Proximal (<10kb) | 544 | |
| chr11:32,436,719–32,437,235 | 5.9 kb | Proximal (<10kb) | 58 | |
| chr11:32,437,732–32,438,388 | 6.9 kb | Proximal (<10kb) | 155 | |
| chr11:32,438,809–32,439,407 | 8.0 kb | Proximal (<10kb) | 120 | |
| chr11:32,441,013–32,441,260 | 5.4 kb | Proximal (<10kb) | 49 |
Genomic view of the WT1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.