The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for WNT4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = WNT4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of WNT4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:21,922,465–21,923,180 | 220.3 kb | Distal (>10kb) Multiome | 263 | |
| chr1:21,936,519–21,938,007 | 205.7 kb | Distal (>10kb) Multiome | 630 | |
| chr1:22,024,858–22,026,137 | 117.6 kb | Distal (>10kb) Multiome | 937 | |
| chr1:22,052,314–22,053,858 | 90.4 kb | Distal (>10kb) Multiome | 912 | |
| chr1:22,142,284–22,143,888 | 253 bp | At TSS Multiome | 298 | |
| chr1:22,154,320–22,155,232 | 11.8 kb | Distal (>10kb) Multiome | 103 | |
| chr1:22,442,329–22,443,072 | 299.5 kb | Distal (>10kb) Multiome | 41 |
Genomic view of the WNT4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.