WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for WIPI2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = WIPI2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of WIPI2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:4,973,785–4,974,732 | 216.1 kb | Distal (>10kb) Multiome | 674 | |
| chr7:5,045,501–5,046,448 | 144.4 kb | Distal (>10kb) Multiome | 594 | |
| chr7:5,189,698–5,190,896 | 12 bp | At TSS Multiome | 725 | |
| chr7:5,240,214–5,240,895 | 50.2 kb | Distal (>10kb) Multiome | 613 | |
| chr7:5,296,049–5,297,455 | 106.6 kb | Distal (>10kb) Multiome | 191 | |
| chr7:5,401,296–5,401,948 | 211.4 kb | Distal (>10kb) Multiome | 104 | |
| chr7:5,406,932–5,408,289 | 217.5 kb | Distal (>10kb) Multiome | 281 | |
| chr7:5,412,740–5,413,653 | 223.1 kb | Distal (>10kb) Multiome | 341 | |
| chr7:5,417,700–5,419,525 | 228.7 kb | Distal (>10kb) Multiome | 714 | |
| chr7:5,423,102–5,425,439 | 233.9 kb | Distal (>10kb) Multiome | 834 | |
| chr7:5,426,416–5,429,142 | 236.8 kb | Distal (>10kb) Multiome | 1072 | |
| chr7:5,450,591–5,451,143 | 260.7 kb | Distal (>10kb) Multiome | 306 | |
| chr7:5,486,725–5,487,360 | 296.8 kb | Distal (>10kb) Multiome | 621 |
Genomic view of the WIPI2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.