This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for VRK1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = VRK1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of VRK1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:96,501,754–96,503,092 | 295.0 kb | Distal (>10kb) Multiome | 986 | |
| chr14:96,583,910–96,584,894 | 213.0 kb | Distal (>10kb) Multiome | 345 | |
| chr14:96,592,395–96,593,217 | 204.6 kb | Distal (>10kb) Multiome | 226 | |
| chr14:96,665,756–96,666,415 | 131.3 kb | Distal (>10kb) Multiome | 41 | |
| chr14:96,693,176–96,694,444 | 103.8 kb | Distal (>10kb) Multiome | 166 | |
| chr14:96,741,173–96,742,080 | 55.7 kb | Distal (>10kb) Multiome | 370 | |
| chr14:96,797,112–96,798,108 | 30 bp | At TSS Multiome | 724 | |
| chr14:97,032,924–97,034,121 | 236.1 kb | Distal (>10kb) Multiome | 180 |
Genomic view of the VRK1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.