Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP24 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 [PubMed 14715245]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for USP24. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = USP24 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of USP24, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:54,950,380–54,951,143 | 264.6 kb | Distal (>10kb) Multiome | 459 | |
| chr1:54,980,478–54,981,190 | 234.6 kb | Distal (>10kb) Multiome | 185 | |
| chr1:54,996,310–54,996,725 | 218.7 kb | Distal (>10kb) Multiome | 355 | |
| chr1:55,021,822–55,022,741 | 193.2 kb | Distal (>10kb) Multiome | 538 | |
| chr1:55,039,084–55,041,196 | 175.7 kb | Distal (>10kb) Multiome | 743 | |
| chr1:55,197,062–55,197,632 | 18.1 kb | Distal (>10kb) Multiome | 178 | |
| chr1:55,206,347–55,206,543 | 8.8 kb | Proximal (<10kb) | 30 | |
| chr1:55,213,795–55,216,078 | 22 bp | At TSS Multiome | 933 | |
| chr1:55,232,539–55,233,863 | 17.9 kb | Distal (>10kb) Multiome | 196 | |
| chr1:55,313,810–55,314,345 | 98.8 kb | Distal (>10kb) Multiome | 208 | |
| chr1:55,337,950–55,338,809 | 123.1 kb | Distal (>10kb) Multiome | 141 | |
| chr1:56,811,001–56,811,706 | 1596.1 kb | Distal (>10kb) Multiome HiCAR | 234 | |
| chr1:56,818,824–56,820,020 | 1604.1 kb | Distal (>10kb) Multiome HiCAR | 480 |
Genomic view of the USP24 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.