The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for UBE2M. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = UBE2M upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of UBE2M, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:58,278,271–58,279,774 | 280.1 kb | Distal (>10kb) Multiome | 950 | |
| chr19:58,304,887–58,305,868 | 253.6 kb | Distal (>10kb) Multiome | 858 | |
| chr19:58,326,789–58,327,710 | 231.9 kb | Distal (>10kb) Multiome | 985 | |
| chr19:58,347,086–58,347,987 | 211.3 kb | Distal (>10kb) Multiome | 467 | |
| chr19:58,356,174–58,356,775 | 202.5 kb | Distal (>10kb) Multiome | 101 | |
| chr19:58,361,859–58,363,243 | 196.2 kb | Distal (>10kb) Multiome | 972 | |
| chr19:58,380,269–58,381,636 | 177.9 kb | Distal (>10kb) Multiome | 1044 | |
| chr19:58,386,311–58,387,531 | 171.9 kb | Distal (>10kb) Multiome | 1010 | |
| chr19:58,395,438–58,396,606 | 163.3 kb | Distal (>10kb) Multiome | 448 | |
| chr19:58,400,803–58,401,800 | 157.6 kb | Distal (>10kb) Multiome | 728 | |
| chr19:58,408,032–58,409,025 | 150.4 kb | Distal (>10kb) Multiome | 854 | |
| chr19:58,451,175–58,452,075 | 107.4 kb | Distal (>10kb) Multiome | 702 | |
| chr19:58,466,585–58,467,517 | 92.0 kb | Distal (>10kb) Multiome | 701 | |
| chr19:58,475,442–58,476,812 | 83.0 kb | Distal (>10kb) Multiome | 934 | |
| chr19:58,498,641–58,499,856 | 59.5 kb | Distal (>10kb) Multiome | 939 | |
| chr19:58,513,642–58,514,431 | 44.8 kb | Distal (>10kb) Multiome | 706 | |
| chr19:58,518,851–58,520,372 | 39.2 kb | Distal (>10kb) Multiome | 1175 | |
| chr19:58,538,071–58,538,713 | 20.4 kb | Distal (>10kb) Multiome | 386 |
Genomic view of the UBE2M locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.