The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2009]
Transcription factors with Perturb-seq knockdown data for UBE2L3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = UBE2L3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of UBE2L3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:21,567,510–21,568,436 | 1 bp | At TSS Multiome | 951 | |
| chr22:21,568,648–21,569,092 | 935 bp | At TSS | 302 | |
| chr22:21,628,821–21,630,259 | 62.4 kb | Distal (>10kb) Multiome | 1055 | |
| chr22:21,641,511–21,643,313 | 74.4 kb | Distal (>10kb) Multiome HiCAR | 754 | |
| chr22:21,651,622–21,652,445 | 84.4 kb | Distal (>10kb) Multiome | 719 | |
| chr22:21,657,073–21,658,429 | 89.9 kb | Distal (>10kb) Multiome | 802 | |
| chr22:21,665,721–21,666,577 | 98.4 kb | Distal (>10kb) Multiome | 893 | |
| chr22:21,735,345–21,736,601 | 168.2 kb | Distal (>10kb) Multiome | 774 | |
| chr22:21,864,937–21,865,391 | 297.3 kb | Distal (>10kb) Multiome | 184 | |
| chr22:21,866,936–21,868,402 | 300.1 kb | Distal (>10kb) Multiome | 929 |
Genomic view of the UBE2L3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.