The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for UBE2J2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = UBE2J2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of UBE2J2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:975,960–976,521 | 297.6 kb | Distal (>10kb) Multiome | 476 | |
| chr1:998,350–1,000,999 | 273.5 kb | Distal (>10kb) Multiome | 1047 | |
| chr1:1,001,349–1,002,099 | 271.9 kb | Distal (>10kb) Multiome | 646 | |
| chr1:1,005,015–1,005,702 | 268.6 kb | Distal (>10kb) Multiome | 532 | |
| chr1:1,019,142–1,021,414 | 254.3 kb | Distal (>10kb) Multiome | 707 | |
| chr1:1,032,438–1,034,753 | 240.6 kb | Distal (>10kb) Multiome | 475 | |
| chr1:1,040,071–1,041,597 | 232.9 kb | Distal (>10kb) Multiome | 410 | |
| chr1:1,058,856–1,060,114 | 214.3 kb | Distal (>10kb) Multiome | 609 | |
| chr1:1,068,556–1,069,991 | 204.6 kb | Distal (>10kb) Multiome | 577 | |
| chr1:1,079,478–1,080,558 | 193.8 kb | Distal (>10kb) Multiome | 746 | |
| chr1:1,115,769–1,117,022 | 157.6 kb | Distal (>10kb) Multiome | 743 | |
| chr1:1,157,424–1,158,362 | 116.3 kb | Distal (>10kb) Multiome | 575 | |
| chr1:1,200,907–1,201,739 | 72.5 kb | Distal (>10kb) Multiome | 430 | |
| chr1:1,231,448–1,233,390 | 41.7 kb | Distal (>10kb) Multiome | 884 | |
| chr1:1,273,333–1,274,645 | 99 bp | At TSS Multiome | 779 | |
| chr1:1,293,434–1,294,454 | 20.0 kb | Distal (>10kb) Multiome | 234 | |
| chr1:1,307,375–1,310,219 | 34.5 kb | Distal (>10kb) Multiome | 897 | |
| chr1:1,324,278–1,325,394 | 50.9 kb | Distal (>10kb) Multiome | 735 | |
| chr1:1,348,716–1,350,027 | 75.7 kb | Distal (>10kb) Multiome | 539 | |
| chr1:1,353,403–1,354,425 | 80.0 kb | Distal (>10kb) Multiome | 276 | |
| chr1:1,374,767–1,375,798 | 101.5 kb | Distal (>10kb) Multiome | 1113 | |
| chr1:1,398,861–1,399,955 | 125.6 kb | Distal (>10kb) Multiome | 879 | |
| chr1:1,406,992–1,407,823 | 133.5 kb | Distal (>10kb) Multiome | 938 | |
| chr1:1,435,318–1,436,066 | 161.8 kb | Distal (>10kb) Multiome | 511 | |
| chr1:1,511,442–1,512,404 | 238.2 kb | Distal (>10kb) Multiome | 897 | |
| chr1:1,540,164–1,541,317 | 266.8 kb | Distal (>10kb) Multiome | 195 |
Genomic view of the UBE2J2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.