The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for UBE2J1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = UBE2J1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of UBE2J1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:89,079,845–89,082,207 | 272.2 kb | Distal (>10kb) Multiome | 1155 | |
| chr6:89,117,325–89,118,595 | 234.7 kb | Distal (>10kb) Multiome | 482 | |
| chr6:89,145,740–89,146,828 | 206.5 kb | Distal (>10kb) Multiome | 853 | |
| chr6:89,242,677–89,243,345 | 109.7 kb | Distal (>10kb) Multiome | 160 | |
| chr6:89,311,590–89,312,158 | 40.8 kb | Distal (>10kb) Multiome | 214 | |
| chr6:89,352,138–89,353,410 | 74 bp | At TSS Multiome | 882 | |
| chr6:89,354,071–89,354,274 | 1.3 kb | Proximal (<10kb) | 63 | |
| chr6:89,411,134–89,412,806 | 59.3 kb | Distal (>10kb) Multiome | 540 | |
| chr6:89,432,777–89,434,173 | 80.4 kb | Distal (>10kb) Multiome | 524 | |
| chr6:89,483,120–89,483,667 | 130.7 kb | Distal (>10kb) Multiome | 199 | |
| chr6:89,493,449–89,494,745 | 141.4 kb | Distal (>10kb) Multiome | 266 | |
| chr6:89,496,006–89,496,706 | 143.6 kb | Distal (>10kb) Multiome | 166 | |
| chr6:89,522,599–89,523,543 | 170.3 kb | Distal (>10kb) Multiome | 138 | |
| chr6:89,588,903–89,590,722 | 237.6 kb | Distal (>10kb) Multiome | 245 | |
| chr6:89,638,049–89,639,180 | 286.0 kb | Distal (>10kb) Multiome | 783 |
Genomic view of the UBE2J1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.