Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. This gene encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]
Transcription factors with Perturb-seq knockdown data for UBE2D2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = UBE2D2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of UBE2D2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:139,273,424–139,274,448 | 287.3 kb | Distal (>10kb) Multiome | 843 | |
| chr5:139,283,068–139,284,092 | 277.7 kb | Distal (>10kb) Multiome | 194 | |
| chr5:139,293,312–139,294,807 | 267.6 kb | Distal (>10kb) Multiome | 994 | |
| chr5:139,341,351–139,342,686 | 219.5 kb | Distal (>10kb) Multiome | 1002 | |
| chr5:139,378,115–139,378,885 | 182.9 kb | Distal (>10kb) Multiome | 122 | |
| chr5:139,391,978–139,393,556 | 168.6 kb | Distal (>10kb) Multiome | 424 | |
| chr5:139,396,045–139,397,275 | 164.5 kb | Distal (>10kb) Multiome | 134 | |
| chr5:139,403,383–139,404,694 | 157.3 kb | Distal (>10kb) Multiome | 772 | |
| chr5:139,438,861–139,439,913 | 121.9 kb | Distal (>10kb) Multiome | 726 | |
| chr5:139,517,739–139,518,561 | 43.2 kb | Distal (>10kb) Multiome | 1046 | |
| chr5:139,523,548–139,524,678 | 37.1 kb | Distal (>10kb) Multiome | 320 | |
| chr5:139,560,824–139,562,261 | 52 bp | At TSS Multiome | 970 | |
| chr5:139,637,254–139,638,208 | 76.3 kb | Distal (>10kb) Multiome | 890 | |
| chr5:139,646,902–139,649,971 | 87.2 kb | Distal (>10kb) Multiome | 723 | |
| chr5:139,668,261–139,668,827 | 107.1 kb | Distal (>10kb) Multiome | 388 | |
| chr5:139,696,560–139,698,449 | 136.1 kb | Distal (>10kb) Multiome | 789 | |
| chr5:139,698,576–139,699,204 | 137.6 kb | Distal (>10kb) Multiome | 291 | |
| chr5:139,700,801–139,701,790 | 140.1 kb | Distal (>10kb) Multiome | 520 | |
| chr5:139,708,355–139,712,383 | 148.2 kb | Distal (>10kb) Multiome | 1075 | |
| chr5:139,745,172–139,748,271 | 185.0 kb | Distal (>10kb) Multiome | 872 | |
| chr5:139,755,957–139,756,675 | 194.9 kb | Distal (>10kb) Multiome | 431 | |
| chr5:139,765,531–139,766,197 | 204.5 kb | Distal (>10kb) Multiome | 676 | |
| chr5:139,774,955–139,775,575 | 213.9 kb | Distal (>10kb) Multiome | 435 | |
| chr5:139,787,176–139,788,865 | 226.5 kb | Distal (>10kb) Multiome | 596 | |
| chr5:139,847,851–139,848,888 | 287.0 kb | Distal (>10kb) Multiome | 252 |
Genomic view of the UBE2D2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.