The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for UBE2B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = UBE2B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of UBE2B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:134,070,573–134,071,963 | 299.9 kb | Distal (>10kb) Multiome | 666 | |
| chr5:134,099,891–134,100,749 | 271.1 kb | Distal (>10kb) Multiome | 227 | |
| chr5:134,101,617–134,102,291 | 269.1 kb | Distal (>10kb) Multiome | 334 | |
| chr5:134,113,043–134,116,112 | 255.5 kb | Distal (>10kb) Multiome | 637 | |
| chr5:134,140,528–134,140,995 | 230.4 kb | Distal (>10kb) Multiome | 342 | |
| chr5:134,176,142–134,177,562 | 194.2 kb | Distal (>10kb) Multiome | 952 | |
| chr5:134,184,086–134,184,650 | 186.8 kb | Distal (>10kb) Multiome HiCAR | 289 | |
| chr5:134,225,248–134,227,248 | 144.9 kb | Distal (>10kb) Multiome | 1043 | |
| chr5:134,366,722–134,367,362 | 4.0 kb | Proximal (<10kb) Multiome | 682 | |
| chr5:134,370,722–134,372,303 | 79 bp | At TSS Multiome | 1080 | |
| chr5:134,411,465–134,412,433 | 40.8 kb | Distal (>10kb) Multiome | 989 | |
| chr5:134,465,626–134,467,213 | 94.8 kb | Distal (>10kb) Multiome | 926 | |
| chr5:134,506,114–134,506,990 | 135.2 kb | Distal (>10kb) Multiome HiCAR | 644 | |
| chr5:134,511,761–134,512,800 | 141.1 kb | Distal (>10kb) Multiome | 505 | |
| chr5:134,523,964–134,528,361 | 156.4 kb | Distal (>10kb) Multiome HiCAR | 1077 | |
| chr5:134,539,671–134,540,206 | 168.7 kb | Distal (>10kb) Multiome HiCAR | 144 | |
| chr5:134,586,828–134,587,543 | 216.0 kb | Distal (>10kb) Multiome HiCAR | 407 | |
| chr5:134,632,348–134,633,007 | 261.6 kb | Distal (>10kb) Multiome | 1019 | |
| chr5:134,648,377–134,649,305 | 277.6 kb | Distal (>10kb) Multiome | 995 |
Genomic view of the UBE2B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.