The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
Transcription factors with Perturb-seq knockdown data for UBE2A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = UBE2A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of UBE2A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chrX:119,399,020–119,399,773 | 175.1 kb | Distal (>10kb) Multiome | 440 | |
| chrX:119,468,031–119,469,511 | 106.2 kb | Distal (>10kb) Multiome | 762 | |
| chrX:119,565,311–119,565,575 | 9.0 kb | Proximal (<10kb) | 451 | |
| chrX:119,573,862–119,575,243 | 35 bp | At TSS Multiome | 734 | |
| chrX:119,605,775–119,606,635 | 31.9 kb | Distal (>10kb) Multiome | 518 | |
| chrX:119,692,568–119,693,792 | 118.8 kb | Distal (>10kb) Multiome | 595 | |
| chrX:119,757,760–119,759,432 | 183.7 kb | Distal (>10kb) Multiome | 507 | |
| chrX:119,791,353–119,792,197 | 217.2 kb | Distal (>10kb) Multiome | 699 | |
| chrX:119,852,659–119,853,400 | 278.5 kb | Distal (>10kb) Multiome | 612 | |
| chrX:119,871,438–119,872,306 | 297.3 kb | Distal (>10kb) Multiome | 720 |
Genomic view of the UBE2A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.