Wybutosine is a hypermodified guanosine found in phenylalanine tRNA. Wybutosine functions to stabilize codon-anticodon interactions during ribosome decoding and therefore supports the maintenance of the reading frame. In yeast, the homolog of this gene is essential for the synthesis of wybutosine. The human genome contains two closely related genes that putatively function in wybutosine synthesis. The open reading frame of this locus is disrupted in some individuals. Thus, this locus appears to be an evolving pseudogene, but may still be functional in some members of the population. [provided by RefSeq, Apr 2014]
Transcription factors with Perturb-seq knockdown data for TYW1B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TYW1B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TYW1B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:72,827,875–72,828,778 | 81 bp | At TSS Multiome | 475 | |
| chr7:72,829,057–72,830,233 | 1.3 kb | Proximal (<10kb) Multiome | 511 | |
| chr7:72,879,029–72,879,545 | 51.1 kb | Distal (>10kb) Multiome | 361 | |
| chr7:72,924,558–72,925,695 | 96.9 kb | Distal (>10kb) Multiome | 687 | |
| chr7:73,005,475–73,006,421 | 177.7 kb | Distal (>10kb) Multiome | 385 |
Genomic view of the TYW1B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.