Wybutosine (yW) is a hypermodified guanosine found in phenylalanine tRNA adjacent to the anticodon that stabilizes codon-anticodon interactions in the ribosome. In yeast, the homolog of this gene is essential for the synthesis of wybutosine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Transcription factors with Perturb-seq knockdown data for TYW1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TYW1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TYW1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:66,740,305–66,741,524 | 256.2 kb | Distal (>10kb) Multiome | 898 | |
| chr7:66,844,266–66,845,342 | 151.9 kb | Distal (>10kb) Multiome | 851 | |
| chr7:66,920,765–66,921,508 | 75.7 kb | Distal (>10kb) Multiome | 957 | |
| chr7:66,994,801–66,994,996 | 1.8 kb | Proximal (<10kb) | 159 | |
| chr7:66,995,101–66,995,823 | 1.3 kb | Proximal (<10kb) Multiome | 496 | |
| chr7:66,996,514–66,997,168 | 56 bp | At TSS Multiome | 407 | |
| chr7:67,099,256–67,099,739 | 102.7 kb | Distal (>10kb) Multiome | 33 | |
| chr7:67,179,175–67,179,622 | 182.6 kb | Distal (>10kb) Multiome | 83 |
Genomic view of the TYW1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.