Transcription factors with Perturb-seq knockdown data for TYMSOS. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TYMSOS upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TYMSOS, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr18:267,462–268,836 | 390.3 kb | Distal (>10kb) Multiome HiCAR | 1007 | |
| chr18:499,984–501,449 | 157.5 kb | Distal (>10kb) Multiome | 306 | |
| chr18:516,064–516,762 | 141.9 kb | Distal (>10kb) Multiome | 96 | |
| chr18:596,942–597,511 | 61.3 kb | Distal (>10kb) Multiome | 522 | |
| chr18:653,638–653,917 | 3.7 kb | Proximal (<10kb) | 21 | |
| chr18:657,152–658,975 | 125 bp | At TSS Multiome | 968 | |
| chr18:685,991–687,114 | 28.3 kb | Distal (>10kb) Multiome | 127 | |
| chr18:712,215–713,458 | 54.5 kb | Distal (>10kb) Multiome | 797 | |
| chr18:811,520–813,366 | 154.2 kb | Distal (>10kb) Multiome | 893 | |
| chr18:906,154–908,403 | 248.5 kb | Distal (>10kb) Multiome | 273 |
Genomic view of the TYMSOS locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.