The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved actin monomer-binding protein. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for TWF2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TWF2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TWF2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:51,941,340–51,942,909 | 297.0 kb | Distal (>10kb) Multiome | 890 | |
| chr3:51,955,723–51,956,827 | 282.6 kb | Distal (>10kb) Multiome | 289 | |
| chr3:51,967,071–51,968,598 | 271.6 kb | Distal (>10kb) Multiome | 581 | |
| chr3:51,973,571–51,975,404 | 265.1 kb | Distal (>10kb) Multiome | 886 | |
| chr3:51,982,506–51,983,633 | 255.9 kb | Distal (>10kb) Multiome | 933 | |
| chr3:51,990,597–51,991,772 | 248.1 kb | Distal (>10kb) Multiome | 145 | |
| chr3:51,995,468–51,996,121 | 243.2 kb | Distal (>10kb) Multiome | 938 | |
| chr3:52,001,516–52,001,992 | 237.5 kb | Distal (>10kb) Multiome | 448 | |
| chr3:52,055,881–52,058,048 | 181.7 kb | Distal (>10kb) Multiome | 937 | |
| chr3:52,128,194–52,128,700 | 110.8 kb | Distal (>10kb) Multiome | 376 | |
| chr3:52,153,766–52,155,196 | 84.5 kb | Distal (>10kb) Multiome | 896 | |
| chr3:52,197,554–52,198,856 | 41.1 kb | Distal (>10kb) Multiome | 731 | |
| chr3:52,231,607–52,231,792 | 7.3 kb | Proximal (<10kb) | 286 | |
| chr3:52,237,653–52,238,416 | 714 bp | At TSS | 535 | |
| chr3:52,238,767–52,239,716 | 98 bp | At TSS Multiome | 763 | |
| chr3:52,245,446–52,246,450 | 6.8 kb | Proximal (<10kb) Multiome | 553 | |
| chr3:52,277,664–52,279,184 | 39.6 kb | Distal (>10kb) Multiome | 844 | |
| chr3:52,287,499–52,288,336 | 48.8 kb | Distal (>10kb) Multiome | 778 | |
| chr3:52,409,430–52,411,187 | 171.2 kb | Distal (>10kb) Multiome | 813 | |
| chr3:52,444,941–52,445,411 | 206.0 kb | Distal (>10kb) Multiome | 426 | |
| chr3:52,455,213–52,455,878 | 216.4 kb | Distal (>10kb) Multiome | 630 | |
| chr3:52,518,607–52,519,389 | 279.8 kb | Distal (>10kb) Multiome | 176 | |
| chr3:52,533,486–52,535,316 | 294.8 kb | Distal (>10kb) Multiome | 648 | |
| chr3:52,536,232–52,537,450 | 297.4 kb | Distal (>10kb) Multiome | 901 |
Genomic view of the TWF2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.