Transcription factors with Perturb-seq knockdown data for TTC32. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TTC32 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TTC32, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:19,863,073–19,864,223 | 38.0 kb | Distal (>10kb) Multiome | 720 | |
| chr2:19,868,540–19,869,572 | 32.7 kb | Distal (>10kb) Multiome | 302 | |
| chr2:19,901,191–19,902,460 | 167 bp | At TSS Multiome | 1066 | |
| chr2:19,989,520–19,990,653 | 88.5 kb | Distal (>10kb) Multiome | 924 | |
| chr2:20,011,873–20,013,148 | 111.0 kb | Distal (>10kb) Multiome | 585 | |
| chr2:20,050,661–20,052,578 | 150.3 kb | Distal (>10kb) Multiome | 1029 | |
| chr2:20,097,914–20,098,555 | 196.7 kb | Distal (>10kb) Multiome | 480 | |
| chr2:20,106,361–20,107,468 | 205.3 kb | Distal (>10kb) Multiome | 181 |
Genomic view of the TTC32 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.