Transcription factors with Perturb-seq knockdown data for TSPOAP1-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TSPOAP1-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TSPOAP1-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:58,082,555–58,084,952 | 241.2 kb | Distal (>10kb) Multiome | 835 | |
| chr17:58,156,945–58,157,756 | 167.3 kb | Distal (>10kb) Multiome | 235 | |
| chr17:58,218,800–58,219,654 | 105.2 kb | Distal (>10kb) Multiome | 890 | |
| chr17:58,249,147–58,250,121 | 74.7 kb | Distal (>10kb) Multiome | 737 | |
| chr17:58,324,153–58,325,169 | 43 bp | At TSS Multiome | 274 | |
| chr17:58,325,271–58,325,527 | 800 bp | At TSS | 103 | |
| chr17:58,338,012–58,338,430 | 13.7 kb | Distal (>10kb) Multiome | 559 | |
| chr17:58,339,009–58,339,515 | 14.8 kb | Distal (>10kb) Multiome | 382 | |
| chr17:58,351,751–58,352,762 | 27.9 kb | Distal (>10kb) Multiome | 990 | |
| chr17:58,487,466–58,488,518 | 163.4 kb | Distal (>10kb) Multiome | 222 | |
| chr17:58,513,911–58,515,157 | 190.1 kb | Distal (>10kb) Multiome | 585 | |
| chr17:58,517,239–58,519,377 | 193.5 kb | Distal (>10kb) Multiome | 1140 | |
| chr17:58,531,392–58,532,430 | 207.6 kb | Distal (>10kb) Multiome | 323 |
Genomic view of the TSPOAP1-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.