The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein and is similar in sequence to its family member CD53 antigen. It is known to complex with integrins and other transmembrane 4 superfamily proteins. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for TSPAN4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TSPAN4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TSPAN4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:554,380–556,321 | 288.2 kb | Distal (>10kb) Multiome | 460 | |
| chr11:559,830–562,079 | 282.5 kb | Distal (>10kb) Multiome | 791 | |
| chr11:567,850–569,533 | 274.7 kb | Distal (>10kb) Multiome | 672 | |
| chr11:574,830–576,748 | 267.2 kb | Distal (>10kb) Multiome | 793 | |
| chr11:613,334–614,059 | 229.6 kb | Distal (>10kb) Multiome | 215 | |
| chr11:615,262–616,320 | 227.4 kb | Distal (>10kb) Multiome | 443 | |
| chr11:636,786–637,700 | 206.2 kb | Distal (>10kb) Multiome | 161 | |
| chr11:693,835–696,330 | 147.9 kb | Distal (>10kb) Multiome | 873 | |
| chr11:705,737–706,270 | 137.3 kb | Distal (>10kb) Multiome | 361 | |
| chr11:720,619–721,074 | 122.4 kb | Distal (>10kb) Multiome | 156 | |
| chr11:727,901–728,499 | 115.1 kb | Distal (>10kb) Multiome | 686 | |
| chr11:746,704–748,069 | 96.0 kb | Distal (>10kb) Multiome | 755 | |
| chr11:762,582–763,602 | 80.0 kb | Distal (>10kb) Multiome | 326 | |
| chr11:776,216–778,328 | 65.9 kb | Distal (>10kb) Multiome | 770 | |
| chr11:783,385–783,909 | 59.6 kb | Distal (>10kb) Multiome | 278 | |
| chr11:797,112–798,675 | 45.1 kb | Distal (>10kb) Multiome | 697 | |
| chr11:804,241–805,812 | 38.0 kb | Distal (>10kb) Multiome | 725 | |
| chr11:808,894–810,402 | 33.6 kb | Distal (>10kb) Multiome | 976 | |
| chr11:818,369–820,592 | 23.8 kb | Distal (>10kb) Multiome | 749 | |
| chr11:826,980–828,116 | 15.8 kb | Distal (>10kb) Multiome | 662 | |
| chr11:828,933–831,262 | 12.9 kb | Distal (>10kb) Multiome | 491 | |
| chr11:832,323–833,565 | 10.4 kb | Distal (>10kb) Multiome | 562 | |
| chr11:842,034–843,556 | 615 bp | At TSS Multiome | 794 | |
| chr11:843,773–845,220 | 1.1 kb | Proximal (<10kb) Multiome | 664 | |
| chr11:848,377–848,758 | 5.5 kb | Proximal (<10kb) | 204 | |
| chr11:849,082–850,270 | 6.5 kb | Proximal (<10kb) Multiome | 229 | |
| chr11:910,337–911,591 | 67.6 kb | Distal (>10kb) Multiome | 754 | |
| chr11:925,038–926,264 | 82.5 kb | Distal (>10kb) Multiome | 689 |
Genomic view of the TSPAN4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.