This gene encodes a mitochondrial translation elongation factor. The encoded protein is an enzyme that catalyzes the exchange of guanine nucleotides on the translation elongation factor Tu during the elongation step of mitchondrial protein translation. Mutations in this gene are associated with combined oxidative phosphorylation deficiency-3 syndrome. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Mar 2010]
Transcription factors with Perturb-seq knockdown data for TSFM. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TSFM upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TSFM, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:57,487,577–57,488,336 | 294.8 kb | Distal (>10kb) Multiome | 784 | |
| chr12:57,519,488–57,521,440 | 262.1 kb | Distal (>10kb) Multiome | 1029 | |
| chr12:57,521,541–57,523,658 | 260.1 kb | Distal (>10kb) Multiome | 960 | |
| chr12:57,546,771–57,547,419 | 235.6 kb | Distal (>10kb) Multiome | 623 | |
| chr12:57,549,654–57,550,343 | 232.8 kb | Distal (>10kb) Multiome | 366 | |
| chr12:57,583,383–57,583,948 | 199.2 kb | Distal (>10kb) Multiome | 353 | |
| chr12:57,590,813–57,591,877 | 191.6 kb | Distal (>10kb) Multiome | 738 | |
| chr12:57,604,466–57,605,404 | 177.9 kb | Distal (>10kb) Multiome | 337 | |
| chr12:57,609,052–57,609,957 | 173.4 kb | Distal (>10kb) Multiome | 83 | |
| chr12:57,610,841–57,611,876 | 171.3 kb | Distal (>10kb) Multiome | 558 | |
| chr12:57,621,603–57,622,199 | 160.9 kb | Distal (>10kb) Multiome | 446 | |
| chr12:57,631,837–57,633,369 | 149.6 kb | Distal (>10kb) Multiome | 706 | |
| chr12:57,693,486–57,694,647 | 88.8 kb | Distal (>10kb) Multiome | 757 | |
| chr12:57,726,079–57,726,879 | 56.5 kb | Distal (>10kb) Multiome | 597 | |
| chr12:57,738,612–57,739,176 | 43.8 kb | Distal (>10kb) Multiome | 201 | |
| chr12:57,744,434–57,745,735 | 37.8 kb | Distal (>10kb) Multiome | 822 | |
| chr12:57,751,806–57,753,147 | 30.3 kb | Distal (>10kb) Multiome | 1030 | |
| chr12:57,754,455–57,756,188 | 27.8 kb | Distal (>10kb) Multiome | 616 | |
| chr12:57,756,649–57,757,193 | 25.8 kb | Distal (>10kb) Multiome | 315 | |
| chr12:57,765,020–57,765,720 | 17.4 kb | Distal (>10kb) Multiome HiCAR | 472 | |
| chr12:57,766,452–57,767,514 | 15.7 kb | Distal (>10kb) Multiome HiCAR | 330 | |
| chr12:57,771,528–57,773,018 | 10.6 kb | Distal (>10kb) Multiome HiCAR | 1006 | |
| chr12:57,782,579–57,783,204 | 16 bp | At TSS Multiome | 916 | |
| chr12:57,814,980–57,815,520 | 32.5 kb | Distal (>10kb) Multiome | 117 | |
| chr12:57,844,422–57,847,797 | 64.3 kb | Distal (>10kb) Multiome | 1032 | |
| chr12:57,852,281–57,853,469 | 70.1 kb | Distal (>10kb) Multiome | 295 | |
| chr12:57,865,060–57,865,767 | 82.7 kb | Distal (>10kb) Multiome | 547 | |
| chr12:57,888,522–57,889,064 | 105.8 kb | Distal (>10kb) Multiome | 328 | |
| chr12:57,896,473–57,897,915 | 114.2 kb | Distal (>10kb) Multiome HiCAR | 419 | |
| chr12:57,935,860–57,936,461 | 153.4 kb | Distal (>10kb) Multiome | 650 | |
| chr12:57,940,778–57,942,227 | 158.7 kb | Distal (>10kb) Multiome | 836 |
Genomic view of the TSFM locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.