Transcription factors with Perturb-seq knockdown data for TSBP1-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = TSBP1-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of TSBP1-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:31,958,527–31,959,598 | 296.0 kb | Distal (>10kb) Multiome | 953 | |
| chr6:31,971,421–31,972,872 | 282.8 kb | Distal (>10kb) Multiome | 1059 | |
| chr6:32,048,217–32,048,679 | 206.7 kb | Distal (>10kb) Multiome | 611 | |
| chr6:32,087,265–32,088,160 | 167.3 kb | Distal (>10kb) Multiome | 473 | |
| chr6:32,127,621–32,128,720 | 126.8 kb | Distal (>10kb) Multiome | 683 | |
| chr6:32,129,973–32,130,800 | 124.7 kb | Distal (>10kb) Multiome | 717 | |
| chr6:32,144,531–32,145,041 | 110.3 kb | Distal (>10kb) Multiome | 44 | |
| chr6:32,148,431–32,149,344 | 106.2 kb | Distal (>10kb) Multiome | 145 | |
| chr6:32,153,307–32,155,059 | 101.1 kb | Distal (>10kb) Multiome | 908 | |
| chr6:32,167,399–32,168,187 | 87.0 kb | Distal (>10kb) Multiome | 411 | |
| chr6:32,175,673–32,176,347 | 79.0 kb | Distal (>10kb) Multiome | 654 | |
| chr6:32,177,078–32,178,952 | 77.1 kb | Distal (>10kb) Multiome | 820 | |
| chr6:32,190,108–32,191,167 | 64.7 kb | Distal (>10kb) Multiome | 661 | |
| chr6:32,195,084–32,196,878 | 59.3 kb | Distal (>10kb) Multiome | 1063 | |
| chr6:32,245,005–32,245,222 | 9.9 kb | Proximal (<10kb) | 309 | |
| chr6:32,255,200–32,255,686 | 241 bp | At TSS Multiome | 364 |
Genomic view of the TSBP1-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.